Spiral Plate

FAQs

• How does a spiral plate work?
  Spiral plates are created using a spiral plater which is an apparatus that dispenses logarithmically decreasing volumes of sample onto the surface of a rotating petri dish forming a spiral pattern. ... The spiral plating method can save time and resources by cutting down on additional dilution and plating steps.
• What is spiral plate count method?
  Spiral Plate Method. The spiral plate count (SPLC) method for microorganisms in milk, foods, and cosmetics is an official method of the APHA (2) and the AOAC (3). In this method, a mechanical plater inoculates a rotating agar plate with liquid sample.
• What is the spread plate method used for?
  The spread plate method is a technique to plate a liquid sample containing bacteria so that the bacteria are easy to count and isolate. A successful spread plate will have a countable number of isolated bacterial colonies evenly distributed on the plate.
• How does a standard plate count work?
  The standard plate count method consists of diluting a sample with sterile saline or phosphate buffer diluent until the bacteria are dilute enough to count accurately. ... Thus, the number of colonies should give the number of bacteria that can grow under the incubation conditions employed.
• What are spiral plates used for?
  Spiral platers are used extensively in food safety testing, as well as the pharmaceutical industry. The spiral plating method can save time and resources by cutting down on additional dilution and plating steps.
• What is spiral in microbiology?
  Spiral bacteria, bacteria of spiral (helical) shape, form the third major morphological category of prokaryotes along with the rod-shaped bacilli and round cocci. Spiral bacteria can be subclassified by the number of twists per cell, cell thickness, cell flexibility, and motility.
• What is the purpose of the spread plate technique?
  Spread plate technique is the method of isolation and enumeration of microorganisms in a mixed culture and distributing it evenly. The technique makes it easier to quantify bacteria in a solution.
• What is pour plate technique?
  The pour-plate technique Molten agar cooled to 45°C, is poured into a Petri dish containing a specified amount of the diluted sample. Following the addition of the molten-then cooled agar, the cover is replaced, and the plates gently rotated in a circular motion to achieve uniform distribution of microorganisms.